🐝
Bombus vosnesenskii Tracking
AprilTag Detection Dashboard · University of Portland · PI: Dr. Tara Prestholdt · Summer 2026
✏️ Edit mode — click any highlighted section to edit. Click Save Changes when done.

Research Project Overview

Pollinator Recovery on Portland's Superfund Shoreline:
Tracking Bombus vosnesenskii at the University of Portland

The University of Portland's Franz River Campus sits on the banks of the Portland Harbor Superfund Site - a 10-mile stretch of the lower Willamette River designated by the EPA in 2000 as one of the most contaminated waterways in the Pacific Northwest. Following a decade of active remediation, this project asks a fundamental restoration ecology question: have native pollinators returned to this recovering riparian landscape, and are they functionally connected to the broader campus green space network?

Using a custom-built 8-station AprilTag detection network, we individually identify Bombus vosnesenskii (yellow-faced bumblebee) workers at both the Franz River Campus riparian corridor and the student-run SLUG garden on upper campus, tracking whether bees move between sites, stay in their capture location, or establish patterns that reveal the ecological connectivity - or isolation - of this recovering habitat.

🐝 Bombus vosnesenskii ⚠️ Portland Harbor Superfund Site University of Portland Franz River Campus SLUG Community Garden Peer-reviewed publication goal Summer 2026
2000
EPA Superfund designation year
2012-2014
Active remediation completed
8
AprilTag detection stations
50
Uniquely tagged individuals

Site context & remediation history

🏭 Portland Harbor Superfund Site EPA designated 2000

The Portland Harbor Superfund Site encompasses a 10-mile stretch of the lower Willamette River contaminated by over a century of industrial activity - including shipyards, manufacturing, and chronic urban stormwater runoff. Sediments and soils along this corridor are heavily tainted with polychlorinated biphenyls (PCBs), dichlorodiphenyltrichloroethane (DDT), heavy metals, and other persistent organic pollutants that accumulated from the late 19th century through the 20th century.

Site contamination: PCBs · DDT · heavy metals · persistent organic pollutants accumulated through over a century of shipyard activity, industrial manufacturing, and urban stormwater discharge into the lower Willamette River.

The University of Portland's river-facing property sits directly on this contaminated corridor. In 2006, the university entered into a formal agreement with the EPA to assess and plan remediation of legacy soil and groundwater pollution along the riverbank. Major active remediation - including contaminated soil excavation, heavy metals and PCBs removal, and riverbank capping to prevent further runoff - took place between 2012 and 2014.

Following remediation, the university developed the Franz River Campus, now home to the E.L. Wiegand Environmental Lab, the university boathouse, and restored riparian plantings along the Willamette shoreline. By 2026, the site has had approximately 12 years to begin ecological recovery since active remediation.

📅 Remediation timeline
⚠️
2000
EPA Superfund Designation
The Portland Harbor designated a federal Superfund site. A 10-mile stretch of the lower Willamette River identified as one of the most contaminated waterways in the Pacific Northwest due to over a century of industrial activity, shipyards, and urban runoff.
📋
2006
University–EPA Agreement
University of Portland enters into a formal agreement with the EPA to assess and plan remediation of legacy soil and groundwater pollution along the river-facing campus property.
🚜
2012–2014
Active Remediation
Major soil excavation and site-wide environmental remediation. Contaminated soil tainted with heavy metals and PCBs removed. Riverbank professionally capped to prevent further runoff into the Willamette. Riparian plantings established along the shoreline.
🏛️
2014–present
Franz River Campus Development
University develops the remediated site into the Franz River Campus, including the E.L. Wiegand Environmental Lab and boathouse. Riparian habitat begins passive ecological recovery. Native plant establishment ongoing.
🐝
2026
This Study
~12 years post-remediation. First systematic survey of native bumblebee presence and movement at the Franz River Campus riparian corridor. 8-station AprilTag tracking network deployed across both riparian and upper campus sites.

Research question

🔬 Core research questions
Primary question: Have Bombus vosnesenskii workers colonized the remediated Franz River Campus riparian corridor, and are they remaining within that habitat or ranging to established green spaces on upper campus (SLUG garden)?

This question is addressed through individual bee tracking at both sites simultaneously. By tagging bees captured at the Franz River Campus (lower campus, river's edge) and at the SLUG community garden (upper campus), we can determine:

🌿 Site fidelity
River bees stay at the river's edge - indicating the remediated habitat provides sufficient floral resources to support resident foragers independently. A positive restoration outcome.
🔗 Habitat connectivity
Bees move between both sites - indicating the riparian corridor and SLUG garden function as a connected network. Even partial recovery integrates into the broader urban pollinator landscape.
📊 Asymmetric movement
Movement is directional - e.g. river bees visit SLUG but not vice versa. Reveals relative habitat quality differences and foraging range relative to patch resource availability.

A secondary question addresses whether the Franz River Campus riparian corridor is supporting a self-sustaining B. vosnesenskii population 12 years after active remediation, or whether bee presence is primarily driven by movement from established upper campus habitats.

Why B. vosnesenskii: The yellow-faced bumblebee is a widespread native generalist throughout the Pacific Northwest, making it an ideal indicator species for habitat recovery assessment. Its foraging range, colony structure, and sensitivity to habitat quality are well-documented, providing a strong comparative baseline for interpreting movement data in a restoration context.

Study sites

🗺️ Detection station layout University of Portland campus
Group 1 - Franz River Campus (lower CAMPUS)
Station A · Station B · Station C · Station D

Remediated riparian corridor along the Willamette River. Formerly contaminated with PCBs, heavy metals, and industrial pollutants. Active soil remediation completed 2012–2014. ~12 years of ecological recovery at time of study.
Group 2 - SLUG Garden (upper campus)
Station E · Station F · Station G · Station H

Student-run community garden with established native plantings, vegetable beds, and pollinator-focused landscaping. Serves as the established reference habitat for comparison with the recovering riparian site.
📋 To be added: Native riparian plant species established at the Franz River Campus during and after remediation. This list will document the specific floral resources available to foraging bees at the remediated site and strengthen the ecological link between habitat restoration and pollinator recovery.

The two sites are separated by approximately 0.25 miles and 180 feet of elevation change - a topographic gradient that adds ecological significance to any confirmed inter-site movement, as bees navigating this corridor must traverse both built campus infrastructure and significant elevation.

🏷️ Tagging protocol

Bees are captured by hand net at both sites during peak foraging hours. Each bee is briefly chilled in a freezer then fitted with a unique AprilTag identifier attached to the dorsal thorax with cyanoacrylate gel adhesive.

  • Capture site, date, time, and tag ID recorded in field manifest
  • Up to 564 unique individual IDs per season
  • Posca paint dots used as backup visual identification
  • Tags persist 10-14 days under field conditions
Tag weight is negligible relative to B. vosnesenskii worker mass (~300–500mg). Studies confirm tags of this size do not significantly affect bumblebee flight (Couvillon et al., 2012).
📊 Automated detection

Each station runs continuous AprilTag detection during preset hours. When a tagged bee enters the camera field, the system automatically:

  • Reads the unique AprilTag ID via OpenCV ArUco3 detection
  • Logs tag ID, station, date, and timestamp to CSV
  • Saves a verification image of each detection event
  • Applies 10-second cooldown to prevent duplicate logging

Detection CSVs from all 8 stations sync automatically to a shared Google Sheet each evening, providing real-time data access for the full research team.

Cross-campus movement confirmed when the same tag ID appears at both a Group 1 (river) and Group 2 (SLUG) station within the same field season.
🌍 Research significance

Documenting native pollinator recovery at a Superfund remediation site addresses a significant gap in restoration ecology literature. While vegetation recovery following contaminated site remediation is well-studied, invertebrate and pollinator recolonization timelines are far less documented - particularly in urban riparian environments.

The Franz River Campus setting is ecologically unusual: a remediated industrial shoreline embedded within an active university campus, adjacent to both the Willamette River and a productive student-managed food garden. This configuration allows direct comparison of pollinator activity between a recovering contaminated site and an established urban agricultural habitat within the same landscape.

Findings from this study have direct relevance for:

  • EPA and regulatory assessment of biological recovery indicators at Superfund sites
  • Urban pollinator conservation planning in Portland and similar post-industrial cities
  • University sustainability and campus ecology programs
  • Restoration practitioners seeking native pollinator recolonization benchmarks
  • Future grant applications to USDA NIFA, NFWF, and EPA STAR programs
Publication target: Findings will be submitted for peer-reviewed publication in Urban Ecosystems, Restoration Ecology, or Journal of Applied Ecology. Student researchers contributing substantially to data collection and analysis will be considered for co-authorship.
👥 Student research team

This project is led by Dr. Tara Prestholdt of the University of Portland Biology Department and the Summer Undergraduate Research Experience. Student researchers manage daily station deployment, bee capture and tagging, data collection, and station maintenance under faculty supervision.

Student involvement spans hardware assembly, ecological field protocols, data management, and statistical analysis - providing genuine hands-on experience in restoration ecology research methods, embedded systems, and scientific computing. The project connects Biology, Environmental Science, and the university's sustainability mission through a real-world research problem with publication-quality outcomes.


Technical appendix

⚙️ Detection system specifications for technical reviewers
Hardware — per station (8 total)
ComputeRaspberry Pi 5 1GB · BCM2712 quad-core 2.4GHz
CameraWaveshare IMX219 · 8MP Sony sensor · 120° FOV · MIPI-CSI
Resolution3280 × 2464 full sensor · continuous video capture
StorageSamsung PRO Endurance 64GB MicroSD · endurance-rated
PowerAnker PowerCore 10K · ~6hr field runtime · USB LED keep-alive
EnclosureIP65 hinged weatherproof box · PATIKIL 15.7" gooseneck arm
MountPVC pipe stake · 1/4"-20 brass heat-set insert in box lid
NetworkUPIoT MAC-registered · Pi Connect remote terminal access
Software stack
OSRaspberry Pi OS Lite 64-bit · Bookworm · headless
DetectionOpenCV-contrib 4.13.0 · DICT_APRILTAG_36h11 · ArUco3
Camerapicamera2 · libcamera v0.7.1
Data syncgspread + google-auth · Google Sheets API v4
Auto-startcrontab @reboot · 20s delay · daily sync 0 20 * * *
Source codegithub.com/bedell-up/beetracking
Tag detection parameters
Tag familyAprilTag 36h11 · 587 unique IDs · strong error correction
Printed size10–12mm · minimum reliable size for B. vosnesenskii thorax
Detection height10–15cm · tag appears ~60px in 3280px-wide frame
Detection modeArUco3 · minSideLengthCanonicalImg = 32
Error correctionerrorCorrectionRate = 1.0 · maximum tolerance
Cooldown10 seconds per tag ID · prevents duplicate logging
References

Couvillon, M.J., et al. (2012). Effect of weight on flight performance of bumblebees. Journal of Apicultural Research.
Olson, E., et al. (2011). AprilTag: A robust and flexible visual fiducial system. Proceedings of ICRA 2011.
U.S. Environmental Protection Agency. (2000). Portland Harbor Superfund Site designation. EPA Region 10.
University of Portland Facilities Planning. Franz River Campus remediation documentation. ww1.up.edu/facilitiesplanning/river-campus.html